Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 May 4;20(9):2195. doi: 10.3390/ijms20092195

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Effects of legumain on the migration, proliferation, and apoptosis of HASMCs. (A) The effect of legumain on HASMC migration was determined in 10 cells per well using a BIOREVO BZ-9000 microscope. Three independent experiments were performed (n = 30). * p < 0.0001. Analyzed by unpaired Student’s t test. (B) The effect of legumain on HASMC proliferation was determined by WST-8 assay. Three independent experiments were performed (n = 3). (C) The effect of legumain on HASMC apoptosis was evaluated by detecting apoptotic cells (green) using a terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-biotin nick end-labeling (TUNEL) assay. Nuclei were co-stained with 6-diamidino-2-phenylindole (blue). The graph indicates the percentage of apoptotic cells in three independent experiments (n = 3). Scale bar = 100 μm.