Figure 7.

Overexpression of HIF-1α attenuates fucoidan-mediated suppression of cell invasion and migration. (A) Breast cancer cells were transfected with HIF-1α overexpression plasmid (HIF-1α-pcDNA3.0). Cell lysates from different groups were prepared and probed for HIF-1α and its target genes, including TWIST and Snail, through western blotting. (B) HIF-1α mRNA expression in breast cancer cells was measured by reverse transcription-quantitative polymerase chain reaction. GAPDH was used as s reference. Post-transfection with HIF-1α-pcDNA3.0, the mRNA expression levels of HIF-1α were increased. Data are presented as the means ± standard deviation. ^aP<0.05 vs. empty plasmid. (C) Scratch assay (magnification, ×100). (D) Transwell assay (magnification, ×200). Breast cancer cells were transfected with HIF-1α-pcDNA3.0 and further treated with 25 µg/ml fucoidan. Migration and invasion of cancer cells were detected after 24 h. Data are presented as the means ± standard deviation. *P<0.05 vs. fucoidan + empty plasmid. CAIX, carbonic anhydrase IX; GLUT-1, glucose transporter protein type-1; HIF-1α, hypoxia-inducible factor-1α.