Figure 2.

Citrate induces G₂/M phase arrest of human PSC cells. (A,B) After treatment of the cells with vehicle (−) or citrate (10 mM) for 36 h, the levels of the indicated proteins in the cell lysates and cell cycle phase were determined using Western blot analysis with specific antibodies and flow cytometric analysis of PI-stained cells, respectively. The values are presented as the means ± standard error of three independent experiments. β-Actin was used as an internal control for sample loading.