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. 2019 May 8;20(9):2264. doi: 10.3390/ijms20092264

Figure 1.

Figure 1

Western blot protein expression analysis of GALNT3 KO and GALNT3/6 KO clones. (A) The CRISPR/Cas9 system was used to generate GALNT3 KO (GALNT3 KO) clones in the A2780s cell line. Five clones show complete protein ablation upon GALNT3 KO (a). Similarly, Western blot confirms the compensation by GALNT6 in the GALNT3 KO clones (b). (B) Western blot analysis of the double KO clones (GALNT3/T6 KO-1 and GALNT3/T6 KO-2) generated using the CRISPR/Cas9 system followed by the shRNA system; mock transfected A2780s cells were used as the control clone (A2780s Ctrl). FN1 was also evaluated in these clones, confirming the activity and expression of GALNT6 in the single GALNT3 KO clones. (C) Western blot confirmation of shRNA-mediated GALNT6 KD in the CaOV3 cell line, in addition to GALNT3 protein expression analysis in the CaOV3 Ctrl and GALNT6 KD clones. β-actin was used as the loading control.