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. 2019 May 8;20(9):2264. doi: 10.3390/ijms20092264

Figure 6.

Figure 6

In vivo examination of the effects of GALNT3 and GALNT3/T6 KO in tumor formation and survival in severe combined immunodeficient (SCID) mice. (A) Survival curves for mice injected with the Ctrl, GALNT3 KO, and GALNT3/T6 KO EOC cells. Median survival of mice injected with Ctrl cells (32 days, n = 7). Survival of mice injected with the GALNT3 KO cells was significantly improved compared with the vector control (p = 0.0046, log-rank test), and importantly, mice injected with the GALNT3/T6 KO cells displayed significantly higher survival rates compared with mice injected with Ctrl cells (p = 0.0002, log-rank test). (B) Tumor weights in SCID mice were measured. Data shown represent the tumor weight averages from seven mice injected with Ctrl cells, eight animals injected with GALNT3 KO cells and seven animals injected with GALNT3/T6 KO cells. Data are presented as mean ± SEM and were analyzed by one-way ANOVA followed by a Dunnett’s test for multiple comparisons. A significant association was considered when p-values were <0.05. GraphPad style was used for p-value presentation; p-value symbols refer as follows: ns p > 0.05, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. (C) Representative immunohistochemistry images of GALNT3, GALNT6, FN1, MUC1, and Ki-67 expression in tumor tissues extracted from mice injected with the Ctrl, GALNT3 KO, and GALNT3/T6 KO cells at 40× (scale bar 60 μm).