Table A1.
Primers used, and quantitative PCR (qPCR) and semi-quantitative PCR (sq-PCR).
| qPCR Primers | ||||
|---|---|---|---|---|
| Gene | Primer | Primer Sequence | Product Size (bp) | Genbank Accession # |
| GALNT3 | Forward | 5′- CAATCAAGGAGGCAAACCAT -3′ | 266 | NM_004482.3 |
| Reverse | 5′- GCTCTCCATTTGCTGAAAGG -3′ | |||
| GALNT6 | Forward | 5′- CACCTTGGCGCTTACGAGA -3′ | 71 | NM_007210.3 |
| Reverse | 5′- CCTGGGAATCTGAACAACCCTC -3′ | |||
| MUC1 | Forward | 5′- ATCTCATTGCCTTGGCTGTC -3′ | 216 | NM_001018016.2 |
| Reverse | 5′- CCACTGCTGGGTTTGTGTAA -3′ | |||
| FN1 | Forward | 5′- GAGAGAAGTGGGACCGTCAG -3′ | 165 | NM_001306129.1 |
| Reverse | 5′- TGGCACCGAGATATTCCTTC -3′ | |||
| BMP2 | Forward | 5′- CCTTTTACTGCCACGGAGAA -3′ | 215 | NM_001200.3 |
| Reverse | 5′- ACAACCCTCCACAACCATGT -3′ | |||
| CFH | Forward | 5′- TGGAAGATGGGATCCAGAAG -3′ | 243 | NM_000186.3 |
| Reverse | 5′- TGAGGTGGTTGTGAACATGG -3′ | |||
| CD36 | Forward | 5′- TGGCTGTGTTTGGAGGTATTC -3′ | 349 | NM_000072.3 |
| Reverse | 5′- AAGTTGTCAGCCTCTGTTCCA -3′ | |||
| ZFHX4 | Forward | 5′- TCAAGTTTGTGCAGCACCTC -3′ | 207 | NM_024721.4 |
| Reverse | 5′- CACGGGATCCTGTCTTCACT -3′ | |||
| THSD7A | Forward | 5′- GCTGGGGCATTTGTGTTACT -3′ | 209 | NM_015204.2 |
| Reverse | 5′- ATTTGTTGTGGCCTCTGGAC -3′ | |||
| CDKN2A | Forward | 5′- ATATGCCTTCCCCCACTACC -3′ | 231 | NM_000077.4 |
| Reverse | 5′- CCCCTGAGCTTCCCTAGTTC -3′ | |||
| TIMP1 | Forward | 5′- AATTCCGACCTCGTCATCAG -3′ | 230 | NM_003254.2 |
| Reverse | 5′- TGCAGTTTTCCAGCAATGAG -3′ | |||
| HGF | Forward | 5′- CTGGTTCCCCTTCAATAGCA -3′ | 168 | NM_000601.5 |
| Reverse | 5′- CTCCAGGGCTGACATTTGAT -3′ | |||
| PCDH9 | Forward | 5′- ATGGCAACTCTGATCCCAAC -3′ | 268 | NM_001318372.1 |
| Reverse | 5′- CGGTCATTGAACTGGTTCCT -3′ | |||
| ID4 | Forward | 5′- TCCGAAGGGAGTGACTAGGA -3′ | 152 | NM_001546.3 |
| Reverse | 5′- CCGAGCCCAACAATTGAC -3′ | |||
| MMP10 | Forward | 5′- AATGAGTTCTGGGCCATCAG -3′ | 151 | NM_002425.2 |
| Reverse | 5′- ATTTGTCCGCTGCAAAGAAG -3′ | |||
| MMP3 | Forward | 5′- TCATTTTGGCCATCTCTTCC -3′ | 155 | NM_002422.4 |
| Reverse | 5′- GGGAAACCTAGGGTGTGGAT -3′ | |||
| TGFBI | Forward | 5′- CTGGTGCGGCTAAAGTCTCT -3′ | 222 | NM_000358.2 |
| Reverse | 5′- CGCTGATGCTTGTTTGAAGA -3′ | |||
| MAL2 | Forward | 5′- ATCCCTGCATGATTTGCATT -3′ | 164 | NM_052886.2 |
| Reverse | 5′- GAGTGTTACGGTCGCCATCT -3′ | |||
| GREM1 | Forward | 5′- GGTATTTGGGTTGAAAGAATTT -3′ | 424 | NM_001191322.1 |
| Reverse | 5′- AAAATACACAACAAATCACATTTTC -3′ | |||
| IFI16 | Forward | 5′- CCTTTCACACTTGTGGCTGA -3′ | 143 | NM_001206567.1 |
| Reverse | 5′- ACCTCAAACACCCCATTCAC -3′ | |||
| CASP4 | Forward | 5′- GCTTTCTGCTCTTCCACACC -3′ | 160 | NM_001225.3 |
| Reverse | 5′- CATCTGGCTGCTCAAATGAA -3′ | |||
| GUSB | Forward | 5′- ATACGTGGTTGGAGAGCTCATT -3′ | 187 | NM_000181.3 |
| Reverse | 5′- CTTGGCTACTGAGTGGGGATAC -3′ | |||
| 18S | Forward | 5′-AACCCGTTGAACCCCATT-3′ | 119 | NR_003278 |
| Reverse | 5′-CCATCCAATCGGTAGTAGCG-3′ |
Note: Forward and reverse primers were designed to recognize different exons of the target gene. The primer positions are based on the reference sequence of the target genes in the Genbank. Expression of 18S and GUSB were used as control for sample normalization in quantification of the gene of interest expression by real-time RT-PCR analysis.