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. 2019 May 7;20(9):2251. doi: 10.3390/ijms20092251

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Intrathecal injection of Abcc8 shRNA decreases mechanical paw withdrawal latencies in mice. (A) Intrathecal injection of AAV9-GFP-U6-m-ABCC8-shRNA (10 μL) significantly decreases mechanical paw withdrawal thresholds over time in mice compared to control injections (AAV9-GFP-U6-m-scramble-shRNA) (repeated measures ANOVA, F (3, 24) = 3.02, p = 0.049). (B) Thermal paw withdrawals were not significantly changed four weeks post injection. (C) Histological images of dorsal root ganglia (DRG), spinal cord and sciatic nerve section from an animal after Abcc8 shRNA injection. Green fluorescent protein is detected in both primary afferent cell bodies and in the spinal cord after five weeks post injection. Images taken at 10× magnification. The mRNA expression of Abcc8 K_ATP channel subunits are decreased in (D) dorsal root ganglia, (E) spinal cord (paired t-test, p = 0.0046) and (F) sciatic nerves (paired t-test, p = 0.045), five weeks after intrathecal injection.