Double immunofluorescence for neuron‐specific enolase (green fluorescence in A and B), neurofilament proteins (green fluorescence in C–F; red fluorescence in G), cytokeratin 20 (red fluorescence in A–F) and Piezo2 (green fluorescence in) in human Merkel's cell‐neurite complexes of subjects with ages of 30), and 88 ) years. Axonal profiles are indicated by arrows. e: epidermis. Objective 63 × /1.40 oil; pinhole 1.37; XY resolution 139.4 nm and Z resolution 235.8 nm. Scale bar: 30 μm (A‐F), 20 μm (B,G).