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. 2019 May 6;18(1):255–264. doi: 10.3892/ol.2019.10326

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Copyright: © Xu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Purification of HeLa-GAPDH from HeLa cell cytosolic proteins by cation-exchange chromatography. (A) HeLa cell cytosolic proteins were separated by cation-exchange chromartography. The proteins were separated by SDS-PAGE and visualized with silver staining. (B) Western blot analysis of proteins from cation-exchange chromatography using healthy donor sera. (C) Western blot analysis where HeLa-GAPDH was detected in fractions from cation-exchange chromatography using anti-human GAPDH polyclonal antibody. HeLa-GAPDH is indicated by arrowheads. M, protein markers; LS, loading sample; FT, flow-through; CW, column wash.