. 2019 May 4;20(9):2196. doi: 10.3390/ijms20092196

Table 3.

Barcode markers developed in this study.

Marker ID	Primer Sequence (5′–3′)	Product Size ^a	Location
Marker ID	Primer Sequence (5′–3′)	Ls/Pj/Gl (bp)	Location
CNV01	F: GGTCAAATACCTAGCGACAA	308/272/254	ycf2
CNV01	R: TTATGCAAGGAGACATTGCT	308/272/254	ycf2
CNV02	F: CATCCATATCCCAATTCCAT	305/371/305	trnN-GUU ~ ycf1
CNV02	R: GCTCGGAGAAGGAAGAGATA	305/371/305	trnN-GUU ~ ycf1
CNV03	F: CATTGAGTGCACCCTATACA	404/386/368	psaC ~ ndhE
CNV03	R: TCGTAACAGAAAATCAACTCG	404/386/368	psaC ~ ndhE
InDel01	F: AACGAATCCTACGGTTTCTC	266/289/269	trnS-GCU ~ trnR-UCU
InDel01	R: TGTCGAACAGGGATAATTTG	266/289/269	trnS-GCU ~ trnR-UCU
InDel02	F: TCTCGCTTTTTAGTCAGTTTG	380/379/416	ndhF ~ rpl32
InDel02	R: GCCTAATGAAAAGCCTAATGA	380/379/416	ndhF ~ rpl32
InDel03	F: CAGGAGGATAGCAAGTTACAA	412/571/571	rps12 ~ trnV-GAC
InDel03	R: CAACGCCACTATTCTTGAAC	412/571/571	rps12 ~ trnV-GAC
InDel04	F: CTATATGTATATACAATAACGAATCA	652/198/633	trnE-UUC ~ trnT-GGU
InDel04	R: GTTCAAGAATAGTGGCGTTG	652/198/633	trnE-UUC ~ trnT-GGU
IR01	LSC P: CCTAGCTGCTGTTGAAGCTC	na/576/na	psbA
	Control F: GACGACTGAGCCAACTTGAT	262/262/262	psbH ~ petB
	Control R: TCGAGACGTTCTTCAAACCA	262/262/262	psbH ~ petB
nrDNA01	Specific F: GTTAACAATTAGGGCGAGCA	na/na/291	ITS1
	Control F: GCATCGATGAAGAACGTAGC	78/78/78	5.8S
	Control R: GCGTTCAAAGACTCGATGGT	78/78/78	5.8S

^a Amplicon size expected for each of the three species. Ls, Ledebouriella seseloides; Pj, Peucedanum japonicum; and Gl, Glehnia littoralis. na, not amplified.