Table A1.
Gene information and specifications for the individual TaqMan (Applied Biosciences) and QGP (Affymetrix) assays used.
| Full Name | Gene Symbol | Chromosomal Location | Taqman Assay ID | GenBank Accession Number 1 |
|---|---|---|---|---|
| Candidate reference genes identified from microarray for NK cells | ||||
| G protein-coupled receptor kinase 2 2 | ADRBK1 | 11q13.2 | Hs00176395_m1 | NM_001619 |
| akirin1 | AKIRIN1 | 1p34.3 | Hs01047800_g1 | NM_024595 |
| protein phosphatase 6 regulatory subunit 3 | PPP6R3 | 11q13.2 | Hs00217759_m1 | NM_018312 |
| Tax1 binding protein 1 | TAX1BP1 | 7p15.2 | Hs00195718_m1 | NM_006024 |
| DnaJ heat shock protein family (Hsp40) member A2 | DNAJA2 | 16q11.2 | Hs00195365_m1 | NM_005880 |
| Candidate reference genes identified from microarray for granulocytes | ||||
| RAB2A, member RAS oncogene family | RAB2A | 8q12.1-q12.2 | na 3 | NM_002865 |
| INSM transcriptional repressor 1 | INSM1 | 20p11.23 | na | NM_002196 |
| YTH N6-methyladenosine RNA binding protein 3 | YTHDF3 | 8q12.3 | na | NM_152758 |
| lymphocyte antigen 6 family member G6E | LY6G6E | 6p21.33 | na | NR_003673 |
| Known endogenous reference genes | ||||
| glyceraldehyde-3-phosphate dehydrogenase | GAPDH | 12p13.31 | na | NM_002046 |
| glucuronidase beta | GUSB | 7q11.21 | Hs00939627_m1 | NM_000181 |
| hypoxanthine phosphoribosyltransferase 1 | HPRT1 | Xq26.2-q26.3 | na | NM_000194 |
| RNA polymerase II subunit A | POLR2A | 17p13.1 | Hs00172187_m1 | na |
| peptidylprolyl isomerase B | PPIB | 15q22.31 | Hs00168719_m1 | NM_000942 |
| Control genes for in vitro cell stimulation | ||||
| prolyl 4-hydroxylase subunit alpha 1 | P4HA1 | 10q22.1 | Hs00914594_m1 | na |
| tumor necrosis factor | TNF | 6p21.33 | Hs00174128_m1 | na |
1 GenBank accession numbers identify QGP assays; 2 At the time of submission, the new symbol for the G protein-coupled receptor kinase 2 gene in the NCBI Entrez database was GRK2. However, the Entrez-based gene definitions for array annotation employed for NK cells used its alias ADRBK1 which was retained unchanged in this work; 3 na, not applicable.