Figure 1.

Experimental design of t(11;14). (A) Schematic illustration of IgH-CCND1 CRISPR/Cas9-mediated induction of t(11;14). (B) gRNA target sequences and tandem-gRNA vector design. (C) Schematic illustration of the IgH-CCND1 fusion gene. CCND1 expression is driven by IgH C region enhancers in B lymphocytes. Two gray arrowheads at the junction (thick gray dotted line) indicate primer positions used for detection of fusion gene. IgH, immunoglobulin heavy chain; CCND1, cyclin D1; Chr, chromosome.