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. 2019 May 2;18(1):275–282. doi: 10.3892/ol.2019.10303

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Copyright: © Tsuyama et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Characterization of IgH-CCND1 CRISPR/Cas9-induced t(11;14). (A) t(11;14) translocation-specific PCR products from cloned cells were directly sequenced. Junctions in the sequence diagrams are indicated by dotted lines. CCND1 and IgH genome sequences included in der(11) and der(14) are indicated by blue (CCND1) and red (IgH) arrows with gRNA sequences. (B) t(11;14)-specific fluorescence in situ hybridization. One of the triploid Chr11 and Chr14 was reciprocally translocated. CCND1 and IgH probes are labeled with red and green boxes, respectively. Scale bar, 5 µm. PAM, protospacer adjacent motif; IgH, immunoglobulin heavy chain; CCND1, cyclin D1; Chr, chromosome.