Table 1.
Number of cytoplasmic structures containing EYFP-GABARAP and EYFP-LC3B identified in SMLM images. Counts are given for the entire size range considered (50 nm–2.8 µm) as well as for two sub-ranges (split alternatively at 100 nm or at 300 nm). For comparison, the numbers of cytoplasmic EYFP-GABARAP and EYFP-LC3B containing structures identified in the corresponding wide-field fluorescence images (named “conventional selection”) are also given.
| Overexpressed Protein | Size Category | Number of Structures | Fraction of Structures (%) |
|---|---|---|---|
| EYFP-GABARAP | 50 nm–2.8 µm | 15,501 | 100 |
| 50 nm–100 nm | 8009 | 51.66 | |
| 100 nm–2.8 µm | 7492 | 48.33 | |
| 50 nm–300 nm | 15,153 | 97.75 | |
| 300 nm–2.8 µm | 348 | 2.25 | |
| Conventional selection | 348 | ||
| EYFP-LC3B | 50 nm–2.8 µm | 18,129 | 100 |
| 50 nm–100 nm | 11,579 | 63.87 | |
| 100 nm–2.8 µm | 6550 | 36.13 | |
| 50 nm–300 nm | 17,643 | 97.32 | |
| 300 nm–2.8 µm | 486 | 2.68 | |
| Conventional selection | 486 |