Table 2.

Molecular characteristics of 24 ESBL-producing E. coli isolates from 21 flies.

Sample No. and Site	Isolate No.	ESBL	Other β-Lactamases	PMQR Genes	Phylogenetic Group	PFGE Type (Clone)	Plasmid Size *
20 H	752/17	CTX-M-1	-	-	A	E1	80 kb
31 H	753/17	SHV-12	-	qnrS1	A	E2	35 kb
32 H	754/17	CTX-M-1	TEM	-	A	E3	160 kb
48 H	755/17	SHV-12	-	qnrS1	A	E2	35 kb
52 H	756/17	CTX-M-15	-	-	B1	E4	n.t.
55 H	757/17	CTX-M-15	-	-	B1	E4	n.t.
61 Z	758/17	CTX-M-1	-	-	A	E5	90 kb
62 Z	759/17	CTX-M-3	-	-	D	E6	80 kb
69 Z	760/17	CTX-M-14	-	-	D	E7	n.t.
71 Z	761/17	CTX-M-14	-	-	D	E7	n.t.
73 Z	762/17	CTX-M-1	-	-	A	E8	n.t.
79 Z	763/17	CTX-M-1	-	-	D	E9	105 kb
84 Z	764/17	CTX-M-15	-	-	D	E10	n.t.
122 RB	765/17	CTX-M-15	TEM	qnrS1	A	E11	n.t.
146 RB	766/17	CTX-M-1	TEM	qnrS1	D	E12	n.t.
153 RB	767/17	CTX-M-1	TEM, LAP	qnrS1	A	E13	n.t.
157 RB	768/17	CTX-M-1	LAP	qnrS1	A	E14	50 kb
157 RB	769/17	CTX-M-1	LAP	qnrS1	A	E14	50 kb
160 RB	770/17	CTX-M-1	TEM	qnrS1	A	E13	n.t.
163 RB	771/17	CTX-M-1	LAP	qnrS1	A	E14	50 kb
163 RB	772/17	CTX-M-1	TEM	qnrS1	D	E12	n.t.
165 RB	773/17	CTX-M-1	LAP	qnrS1	A	E14	50 kb
165 RB	774/17	CTX-M-1	TEM	qnrS1	D	E12	n.t.
166 RB	775/17	CTX-M-1	LAP	qnrS1	A	E14	50 kb

Sites: H—hospital, Z—zoo, RA—residential area A, RB—residential area B; PMQR, plasmid mediated quinolone resistance; * plasmids containing the ESBL gene, transferred using broth mate conjugation assay in E. coli J53 Azi^R; plasmid size was determined using S1-nuclease-PFGE analysis (see Table S1); n.t. not tested—transfer of resistance by both mate conjugation was not successful; PCR amplicons of bla_TEM-like and bla_LAP-like genes were not sequenced.