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. 2019 May 10;20(9):2325. doi: 10.3390/ijms20092325

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Arazyme reduced intracellular lipid accumulation in PA-induced HepG2 cells. (A,B) Viability of arazyme in HepG2 cells treated with arazyme. Cells were incubated with the indicated concentrations of arazyme for 24 h without (A) or with (B) PA. Oil-red O staining images (C, × 200 magnification) and intracellular TG contents (D). (E,F) Protein expression levels of SREBP-1 and FAS were detected by Western blotting. Data are presented as means ± SD (n = 3). ^# p < 0.05, ^## p < 0.01 vs. cells treated with medium only; * p < 0.05, ** p < 0.01 vs. cells treated with PA only by Student’s t-tests.

Arazyme reduced intracellular lipid accumulation in PA-induced HepG2 cells. (A,B) Viability of arazyme in HepG2 cells treated with arazyme. Cells were incubated with the indicated concentrations of arazyme for 24 h without (A) or with (B) PA. Oil-red O staining images (C, × 200 magnification) and intracellular TG contents (D). (E,F) Protein expression levels of SREBP-1 and FAS were detected by Western blotting. Data are presented as means ± SD (n = 3). ^# p < 0.05, ^## p < 0.01 vs. cells treated with medium only; * p < 0.05, ** p < 0.01 vs. cells treated with PA only by Student’s t-tests.