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. 2019 May 1;24(9):1700. doi: 10.3390/molecules24091700

Figure 1.

Figure 1

Effects of TW-37 on cell viability and apoptosis of human mucoepidermoid carcinoma (MEC) cell lines. MC3 and YD-15 cells were treated with dimethyl sulfoxide (DMSO) or a 1.25 μM concentration of TW-37 for 24 h or 48 h. (A) Cell viability was examined by a trypan blue exclusion assay. The results represent the mean ± SD of triplicate from three independent experiments and significant differences are shown (*, p <0.05). (B) Cleavage of poly (ADP-ribose) polymerase (PARP) was analyzed by western blotting and actin was used as a loading control. (C) Nuclear fragmentation and DNA condensation were visualized by fluorescence microscopy. Representative images from three independent experiments are shown (magnification, X400). (D) Fluorescence-activated cell sorting (FACS) analysis of annexin V/ propidium iodide (PI) staining. The annexin V-positive cells are expressed as the percentage of apoptotic cells. (E) FACS analysis of PI staining. The histogram represents the distribution of cell cycles and the graphs represent the mean ± SD from three independent experiments. *, p < 0.05.