. 2019 Apr 28;20(9):2096. doi: 10.3390/ijms20092096

Table 1.

Phosphorylation and Mg²⁺ impacts on CK receptor(rd)–HPt interaction according to Prodigy server calculations.

Protein–Protein Complex	ΔG (kcal/mol)	ΔG (kJ/mol)	K_d (μM) at 25 °C
AHK3rd–AHP2_Apo	−8	−33.47	1.3
AHK3rd–AHP2_Mg²⁺	−8.5	−35.56	0.6
AHK3rd(P) –AHP2_Mg²⁺	−8.8	−36.82	0.35
AHK3rd–AHP2(P)_Mg²⁺	−7.9	−33.05	1.5
AHK3rd(P)–AHP2(P)_Mg²⁺	−8.1	−33.89	1.1
AHK3rd(D941E)–AHP2_Mg²⁺	−8.5	−35.56	0.54
AHK3rd–AHP2(H82E)_Mg²⁺	−8.4	−35.15	0.72

Bold indicates a phosphorylated (P) state of a protein or mimics (D941E, H82E) such a state.