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. 2019 May 11;20(9):2343. doi: 10.3390/ijms20092343

Figure 3.

Figure 3

Coomassie-stained 15% SDS-PAGE of sfGFP(1TAG) and sfGFP(5TAG) constructs purified via Ni-NTA chromatography. (A) sfGFP WT and sfGFP(1TAG) constructs expressed in E. coli BL21(DE3) with different o-pairs (indicated above). (B) sfGFP(5TAG) constructs expressed in E. coli C321.ΔA.exp(DE3) cells with different o-pairs (indicated above). Equal volumes of pooled Ni-NTA elution fractions were used. The expected molecular weight of full-length sfGFP construct is ∼40 kDa (varies slightly with ncAA incorporation), while translation termination at position 2 of the reporter gene construct results in a truncation product ~12.5 kDa in size.