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Stability of MjTyrRS wild type and derived aaRS variants measured by circular dichroism (CD) spectroscopy. Thermal protein denaturation was performed in phosphate buffer while recording the CD signal at 222 nm. Due to the high thermostability of the wild type enzyme, the corresponding Tm value (>95 °C) cannot be accurately determined in the chosen setup. Calculated Tm values for ONBYRS-1 and MjONBYRS are 64.8 °C and 71.0 °C, respectively.
