| aaRS | aminoacyl-tRNA synthetase |
| CAT | chloramphenicol acetyltransferase |
| CD | circular dichroism |
| CED | computational enzyme design |
| Cm | chloramphenicol |
| DOPA | L-3,4-dihydroxyphenylalanine |
| ELP | elastin-like polypeptide |
| Mb | Methanosarcina barkeri |
| Mj | Methanocaldococcus jannaschii |
| Mm | Methanosarcina mazei |
| ncAA | noncanonical amino acids |
| ONB-DOPA | ortho-nitrobenzyl L-3,4-dihydroxyphenylalanine |
| ONBY | ortho-nitrobenzyl L-tyrosine |
| o-pair | orthogonal pair |
| OTS | orthogonal translation system |
| PylRS | pyrrolysyl-tRNA synthetase |
| RF1 | release factor 1 |
| sfGFP | superfolder green fluorescent protein |
| SSM | site-saturation mutagenesis |
| SUMO | small ubiquitin-like modifier |
| Tyr | L-tyrosine |
| TyrRS | tyrosyl-tRNA synthetase |
| WT | wild type |