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Morphine alone is not able to induce NETs. (A) Extracellular DNA from neutrophils was compared following treatment with or without morphine. (B) According to the immunofluorescence results, NETs were absent in the neutrophils stimulated with morphine alone. Magnification, 200×. NETs, neutrophil extracellular traps; Sup, supernatant of LLC cell culture; M, morphine; Cit-H3, citrullinated histone 3.
