Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Comparison of PCR-based techniques. RT-PCR and dPCR using the same amplification reagents and fluorescent labeling system. In dPCR, the sample is first partitioned such that each partition contains either a few or no DNA sequences. Fluorescence is measured at the end of the PCR. In qPCR, the amount of amplified DNA is measured at each cycle during the PCR reaction.
