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. Author manuscript; available in PMC: 2019 Jun 13.
Published in final edited form as: J Am Chem Soc. 2018 May 30;140(23):7092–7100. doi: 10.1021/jacs.7b13488

Figure 3.

Figure 3

Majority of 6AzGlc-dependent labeling is intracellular. (A) Comparison of 6AzGlc to other azide-containing MCRs. NIH3T3 cells were incubated with one of the indicated MCRs (each at 200 μM) or DMSO vehicle for 16 h before CuAAC and in-gel florescence scanning. (B) 6AzGlc is largely excluded from cell-surface glycans. NIH3T3 cells were incubated in triplicate with the indicated MCRs (each at 200 μM) for 16 h. The cells were then harvested and reacted with DBCO-biotin. After incubation with FITC-streptavidin, cell-surface labeling was measured by flow cytometry. Error bars represent ± s.e.m. from the mean of biological replicates (n = 3). The data in all panels is representative of two biological replicates.