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. Author manuscript; available in PMC: 2019 Jun 13.
Published in final edited form as: J Am Chem Soc. 2018 May 30;140(23):7092–7100. doi: 10.1021/jacs.7b13488

Figure 6.

Figure 6

O-GlcNAcase does not remove 6AzGlc labeling. (A) H1299 cells were treated with 200 μM Ac46AzGlc for 5 h, at which time media was exchanged for fresh media containing 10 μM of the OGA inhibitor Thiamet-G or DMSO. Cells were harvested at the times indicated and subjected to CuAAC before being analyzed by in-gel fluorescence scanning. (B) Inhibition of the proteasome stabilizes some 6AzGlc signal. H1299 cells were treated with 200 μM Ac46AzGlc for 5 h, at which time media was exchanged for fresh media containing 10 μM of the proteasome inhibitor MG132 or DMSO. Cells were harvested at the times indicated and subjected to CuAAC before being analyzed by in-gel fluorescence scanning.