Table S1.
Effects of different stabilizers and combination with Tween-80 on particle size, PI and zeta potential of nanosuspensions by media milling preparation method
| Stabilizer | Particle size (nm) | PI | Zeta potential (mV) | |
|---|---|---|---|---|
| PVP K40 | H | 666.0±28.4 | 0.118±0.022 | −19.29±0.85 |
| M | 707.6±13.3 | 0.132±0.027 | −23.35±0.67 | |
| L | 679.6±23.5 | 0.103±0.032 | −23.60±1.05 | |
| PVA | H | 417.5±27.7 | 0.176±0.050 | −25.20±1.06 |
| M | 412.0±32.1 | 0.154±0.011 | −23.52±0.25 | |
| L | - | - | - | |
| F-68 | H | 793.1±62.8* | 0.178±0.034 | −26.57±0.45 |
| M | 915.7±37.3* | 0.154±0.011 | −27.16±1.25 | |
| L | 1,035±110.9 | 0.176±0.050 | −27.06±0.70 | |
| F-108 | H | 301.3±9.50* | 0.162±0.026 | −31.77±1.23 |
| M | 417.9±12.9* | 0.150±0.014 | −25.82±1.29 | |
| L | 578.7±10.1 | 0.198±0.006 | −25.45±0.72 | |
| T-80 | H | 475.4±12.9 | 0.112±0.028 | −32.64±0.64 |
| M | 481.6±22.2 | 0.123±0.051 | −32.73±0.92 | |
| L | 455.4±12.7 | 0.143±0.034 | −28.36±1.56 | |
| PVP K40/Tween-80 | (1 : 1#) | 581.9±31.22 | 0.128±0.049 | −25.32±0.88 |
| PVA/Tween-80 | 414.6±10.72 | 0.164±0.042 | −28.80±1.11 | |
| F-68/Tween-80 | 413.5±22.39 | 0.175±0.053 | −32.56±1.78 | |
| F-108/Tween-80 | 366.2±13.24* | 0.140±0.016 | −31.81±0.96 |
Notes: The ratios of CZ48/stabilizer were 1/1 (H), 1:0.25 (M) and 1:0.1 (L). -, : The drug could not be wetted by the stabilizer. #The amount of stabilizer was 10% weight of CZ48. *p<0.05 by ANOVA and Tukey’s post hoc.
Abbreviations: H, the ratio of stabilizer/CZ48 is 1:1; M, the ratio of stabilizer/CZ48 is 1:4; L, the ratio of stabilizer/CZ48 is 1:10; PVP K40, povidone 40; PVA, polyvinyl acetate; F-68, Pluronic®F68; F-108: Pluronic®F108; Tween-80, polysorbate 80.