TABLE 2.
Summary of significant effects of genotype on dendrite morphology
| Brain region of neurons | Morphology parameter | Sex | Genotypes | Change from WT, % | Genotypes | Change from T4826I, % | Genotypes | Change from DM, % |
|---|---|---|---|---|---|---|---|---|
| Hippocampal | Distance from soma of peak # intersections (μm) | Male | WT vs T4826I | 31.73% increase | DM vs 48261 | 25.28% increase | ||
| Hippocampal | Number of primary dendrites/neuron | Male | WT vs T4826I | 20.68% decrease | T4826I vs CGG | 35.35% increase | DM vs CGG | 21.85% increase |
| Hippocampal | # Dendritic tips/neuron | Male | WT vs CGG | 24.67% increase | T4826I vs CGG | 17.72% increase | DM vs CGG | 27.77% increase |
| Hippocampal | Tips/primary dendrite | Male | WT vs T4826I | 46.49% increase | ||||
| Hippocampal | Mean dendritic length (μm) | Male | WT vs T4826I | 41.23% increase | ||||
| Hippocampal | Soma area (μm2) | Female | WT vs CGG | 22.87 % increase | T4826I vs CGG | 20.81% increase | DM vs CGG | 27.5% increase |
| Hippocampal | Soma area (μm2) | Male | T4826I vs CGG | 18.89% increase | DM vs CGG | 25.39% increase | ||
| Cortical | Distance from soma of peak # intersections (μm) | Female | T4826I vs CGG T4826I vs DM |
16.36% increase 17.5% increase |
||||
| Cortical | Distance from soma of peak # intersections (μm) | Male | WT vs CGG | 16.75% increase | ||||
| Cortical | # Intersections at peak | Female | T4826I vs CGG | 17.94% increase | ||||
| Cortical | Area under sholl curve | Female | T4826I vs DM | 29.99% increase | ||||
| Cortical | Distal area under sholl cuve | Female | WT vs DM | 22.84% increase | ||||
| Cortical | # Dendritic tips/neuron | Female | WT vs DM | 19.79% increase | T4826I vs DM | 23.03% increase | ||
| Cortical | Tips/primary dendrite | Male | WT vs DM | 17.22% increase | T4826I vs DM | 18.42% increase | ||
| Cortical | Tips/primary dendrite | Female | WT vs DM WT vs CGG |
32.07% increase 23.33% increase |
T4826I vs DM | 23.38% increase | ||
| Cortical | Mean dendritic length (μm) | Female | WT vs DM | 47.42% increase | T4826I vs DM | 40.85% increase |
Abbreviations: CGG, mice expressing a CGG repeat expansion in the fragile X mental retardation 1 gene; DM, double mutation (CGG+T4826I); T4826I, T4826I-ryanodine receptor 1 mutation; WT, wild-type.