Table 3.

Reagents and buffers for on-chip isolation of viral RNA targets (i.e., HIV virus)

Reagent step	Qiagen QIAamp™ viral RNAkit	Roche High Pure™ Viral RNA kit	Generic reagents
Lysis/binding buffer	AVL buffer (contains guanidine thiocyanate)	Lysis/binding buffer Inhibitor removal buffer (4.5 M guanidine-HCl, 50 mM Tris–HCl, 30 % Triton® X-100)	6 M Guanidine HCl or 6 M guanidine thiocyanate
Wash buffer 1	AW1 buffer (contains guanidine HCl), ~50 % ethanol	Inhibitor removal buffer (5 M guanidine-HCl, 20 mM Tris–HCl, 40 % (v/v) ethanol, pH 6.6)	50 % ethanol; 50 % water
Wash buffer 2	AW2 buffer, ~50 % ethanol	Wash Buffer (approx. 50 % (v/v) ethanol, 20 mM NaCl, 2 mM Tris–HCl, pH 7.5)	50 % ethanol; 50 % water