Suppression of intracellular lipid accumulation by CG-1. (A) Cytotoxicity of CG-1 in 3T3-L1 cells. The cells were incubated for 6 days in DMEM with various concentrations of CG-1 (0-10 μM). Data show the means ± S.D. from three experiments. (B) Oil Red O staining of intracellular lipid droplets. 3T3-L1 cells (undifferentiated cells: U) were differentiated into adipocytes (D) for 6 days in DMEM with various concentrations of CG-1 (0–10 μM). The cells were observed by a microscope (200x). Scale bar = 200 μm. (C) The intracellular triglyceride level. 3T3-L1 cells (undifferentiated cells: U; white column) were differentiated (D) into adipocytes for 6 days in DMEM without (gray column) or with CG-1 (0.5, 1, 5, 10 μM; black columns). Data are presented as the means ± S.D. from three experiments. * p < 0.01, ** p < 0.05, as indicated by the brackets.