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. 2019 May 5;20(9):2211. doi: 10.3390/ijms20092211

Figure 4.

Figure 4

Effects of CoPP and CORM-2 on the protein levels of HO-1, NOS1, CD11b/c, GFAP, p-ERK 1/2, and p-JNK in the LC of NOS2-KO mice. Protein levels of HO-1 (A), NOS1 (B), CD11b/c (C), GFAP (E), p-ERK 1/2 (F), and p-JNK (G) in the LC of CFA-injected NOS2-KO mice treated with vehicle, CORM-2, or CoPP are represented. These levels from naïve mice treated with vehicle are also represented as controls. In all panels, * denotes significant differences vs. naïve vehicle-treated mice, + represents significant differences vs. CFA-injected mice treated with vehicle, # represents significant differences vs. CFA-injected mice treated with CORM-2, and $ represents significant differences vs. CFA-injected mice treated with CoPP (p < 0.05, one-way ANOVA, Student–Newman–Keuls test). Examples of western blots for HO-1 (32 kDa), NOS1 (160 kDa), CD11b/c (160 kDa), GFAP (50 kDa), GAPDH (37 kDa), p-ERK 1/2/total ERK 1/2 (42–44 kDa), and p-JNK/total JNK (46–54 kDA) are shown (D). The levels of phosphorylated proteins are relative to the total levels of the corresponding proteins, while the levels of the remaining proteins are relative to those of GAPDH. Each column represents the mean, and the vertical bars indicate the SEM; n = 4 samples per group.