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. 2019 Apr 30;18(1):237–246. doi: 10.3892/ol.2019.10297

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Copyright: © Xie et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Silencing efficiency of three siRNA sequences targeting the ERCC6L gene as determined by RT-qPCR. GAPDH was used as an internal control. RT-qPCR analysis of mRNA in (A) SW480 cells and (B) HT29 cells transfected with ERCC6L siRNAs and NC siRNA. Western blot analysis of ERCC6L protein expression in (C) SW480 cells and (D) HT29 cells transfected with an ERCC6L siRNA and NC siRNA. GAPDH served as a loading control. **P<0.01, ***P<0.001. si, small interfering; NC, negative control; ERCC6L, excision repair cross-complementation group 6 like; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.