Table 2.
Linearity, sensitivity, system precision and accuracy (n = 6).
| Phenolic Acids | Equation | R2 | Range (μg/mL) | Average Recovery (Mean ± SD) |
|---|---|---|---|---|
| b Chlorogenic acid | a Y = 1.25 × 106X − 5405.19 | 0.9997 | 3.75–22.50 | 100.5% ± 3.80% |
| Caffeic acid | Y = 4.00 × 106X − 8763.30 | 0.9997 | 2.70–21.60 | 96.7% ± 1.39% |
| Isochlorogenic acid B | Y = 3.09 × 106X − 5324.83 | 0.9999 | 0.95–5.70 | 97.8% ± 2.31% |
| Isochlorogenic acid A | Y = 2.25 × 106X + 18885.5 | 0.9999 | 0.90–11.25 | 100.4% ± 2.81% |
| Isochlorogenic acid C | Y = 2.53 × 106X − 55085.10 | 0.9999 | 8.50–51.00 | 99.7% ± 2.51% |
| caffeic acid phenethyl ester | Y = 2.36 × 106X − 9337.66 | 0.9996 | 12.00–72.00 | 98.5% ± 1.66% |
| Artepillin C | Y = 3.14 × 106X + 63207.59 | 0.9987 | 12.30–98.30 | 99.5% ± 1.32% |
a Response y, is the peak area ratio (area/migration time) of the analytes versus that of standard compound. b Detection was carried out at 310 nm (Chlorogenic acid, Caffeic acid, Isochlorogenic acid B, Isochlorogenic acid A, Isochlorogenic acid C, caffeic acid phenethyl ester, Artepillin C).