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The effect of GA on protein expression of regulators of the cell cycle and epithelial-to-mesenchymal transition (EMT). LNCap (A), DU145 (B), and PC3 (C) cell lines were treated with GA at the indicated concentrations for 48 h. Cell extracts were analyzed by Western blotting with the antibodies of cyclin D1, CDK4, twist, snail, slug, vimentin, and E-cadherin, respectively. All experiments were performed in three independent experiments.
