Table 2.

Performances of the Proposed Meta-4mCpred and Two State-of-Art Predictors, 4mCPred and 4mcPred-SVM, on Six Independent Datasets from Different Species

Species	Predictors	MCC	ACC	Sn	Sp	TP	FN	FP	TN	p Value
C. elegans	4mCPred	0.731	0.865	0.883	0.849	666	84	118	632	0.670
	4mcPred-SVM	0.684	0.842	0.828	0.856	621	129	108	642	0.001*
	Meta-4mCpred	0.741	0.870	0.843	0.897	632	118	77	673	–
D. melanogaster	4mCPred	0.803	0.900	0.933	0.868	933	67	132	868	0.465
	4mcPred-SVM	0.771	0.886	0.886	0.885	886	114	115	885	0.030*
	Meta-4mCpred	0.812	0.906	0.913	0.899	913	87	101	899	–
A. thaliana	4mCPred	0.632	0.816	0.842	0.789	1,053	197	264	986	<0.00001*
	4mcPred-SVM	0.649	0.824	0.842	0.806	1,053	197	242	1,008	<0.00001*
	Meta-4mCpred	0.711	0.855	0.876	0.834	1,095	155	207	1,043	–
E. coli	4mCPred	0.634	0.817	0.851	0.784	114	20	29	105	0.887
	4mcPred-SVM	0.569	0.784	0.746	0.821	100	34	24	110	0.132
	Meta-4mCpred	0.650	0.825	0.806	0.843	108	26	21	113	–
G. subterruneus	4mCPred	0.578	0.789	0.757	0.820	265	85	63	287	<0.00001*
	4mcPred-SVM	0.624	0.811	0.783	0.840	274	76	56	294	<0.00001*
	Meta-4mCpred	0.701	0.850	0.817	0.883	286	64	41	309	–
G. pickeringii	4mCPred	0.503	0.742	0.610	0.875	122	78	25	175	<0.00001*
	4mcPred-SVM	0.515	0.758	0.750	0.765	150	50	47	153	<0.00001*
	Meta-4mCpred	0.700	0.850	0.835	0.865	167	33	27	173	–

MCC, Matthews correlation coefficient; ACC, accuracy; Sn, sensitivity; Sp, specificity; TP, true positive; FN, false negative; FP, false positive; TN, true negative. The last column represents McNemar’s Chi-squared test, which was used to evaluate the performance between Meta-4mCpred and other methods. *A p value < 0.05 was considered to indicate a statistically significant difference between Meta-4mCpred and the selected method.