Fig. 1.
NetB regulates MB lobe extension. a Images of WT, NetAΔ and NetBΔ MBs in adult brains. FasII labeling showed similar lenghth of α/β lobes in WT and NetAΔ, whrease NetBΔ MBs displayed short lobes (arrowhead). b Results from (a) were quantified for the percentage of brain hemispheres with short α/β lobes. (WT, n = 24; NetAΔ, n = 28; netBΔ, n = 33; n indicates fly numbers. **p < 0.01). c MBs from control, NetA RNAi, NetB RNAi, NetA over-expression (OE) and NetB OE were visulized by mCD8-GFP driven by Ok107-Gal4 and immunostaining for FasII. Altered NetA expression caused no neuroanatomical MB defects, wherease NetB knocked-down MBs showed servere short lobes (arrowhead) and NetB OE MBs resulted in overextended α/β lobes (arrow).d The percentage of brain hemispheres with short α/β lobes in NetA/NetB RNAi flies. (OK107-Gal4, n = 30; OK107 > UAS-NetA RNAi, n = 32; OK107 > UAS-NetB RNAi, n = 26; ***p < 0.001). e The percentage of brain hemispheres with overextension of α/β lobes in flies over-expressing Netrins. (OK107-Gal4, n = 30; OK107 > UAS-NetA, n = 30; OK107 > UAS-NetB, n = 28, ***p < 0.001). Significance was determined by Fisher’s exact test. Scale bars: 50 μm