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. 2019 May 28;12:52. doi: 10.1186/s13041-019-0472-1

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — NetB expression pattern and MB phenotypes in NetB RNAi flies during brain development. a-f NetB expression pattern in NetB^tm flies, as revealed by anti-myc staining (gray). a-b Strong expression of NetB was observed in the VNC cells at third Larva stage (L3) and 18 h APF. c At stage of the 24 h APF, NetB was enriched around the end of peduncles of MB and in the ellipsoid body (EB). d-f NetB expression from 36 h APF until adult stage. MB lobes were strongly stained with anti-myc. g-n The MB morphology of the control (OK107 > UAS-mCD8-GFP) and NetB RNAi (UAS-NetB RNAi/+; UAS-mCD8-GFP/+;OK107-Gal4/+)flies at L3,18h APF,27 h APF,36 h APF. Upper line shows the control group MBs and lower line shows the NetB RNAi MBs. Numbers in the upper right corners indicate the numbers of observed MBs in corresponding genotypes