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. 2019 May 22;10:328. doi: 10.3389/fendo.2019.00328

Table 2.

Ubiquitinated proteins identified in nonfunctional pituitary adenomas relative to controls.

Accession No. Gene name Protein name Modified peptides Peptide length Modified positions Modified probabilities Modified level (N) Modified level (T) Ratio (T/N) t-test p-value
A0A0A6YY96 IREB2 Iron-responsive element-binding protein 2 GFQIAAEK*QK* 10 483;485 1; 1 3.59E+07 1.62E+08 4.52 4.55E-06
P16104 H2AFX Histone H2AX K*TSATVGPK 9 120 1 3.50E+07 1.43E+09 40.96 1.39E-05
P48200 IREB2 Iron-responsive element-binding protein 2 GFQIAAEK*QK* 10 483;485 1; 1 3.46E+07 1.63E+08 4.71 3.31E-05
B2RDW1 RPS30A Epididymis luminal protein 112 TLTGK*TITLEVEPSDTIENVK 21 11 1 1.70E+08 1.26E+09 7.40 3.64E-05
TLSDYNIQK*ESTLHLVLR 18 63 1 3.71E+07 1.64E+08 4.43 6.07E-03
LIFAGK*QLEDGR 12 48 1 2.91E+09 7.32E+09 2.52 6.88E-03
IQDK*EGIPPDQQR 13 33 1 2.68E+06 3.68E+07 13.74 9.55E-03
TITLEVEPSDTIENVK*AK 18 27 0.956 1.95E+07
MQIFVK*TLTGK 11 6 0.923
TITLEVEPSDTIENVKAK* 18 29 0.594
VDENGK*ISR 9 113 1
P62979 RPS27A Ubiquitin-40S ribosomal protein S27a TLTGK*TITLEVEPSDTIENVK 21 11 1 1.72E+08 1.27E+09 7.37 3.78E-05
TLSDYNIQK*ESTLHLVLR 18 63 1 3.71E+07 1.96E+08 5.27 6.89E-04
P68871 HBB Hemoglobin subunit beta FFESFGDLSTPDAVMGNPK*VK* 21 60;62 0.5; 0.5 1.69E+06 2.01E+07 11.87 3.71E-04
GTFATLSELHCDK*LHVDPENFR 22 96 1 1.89E+07 9.13E+07 4.83 1.23E-02
K*VLGAFSDGLAHLDNLK 17 67 1 2.07E+06 1.02E+07 4.92 1.22E-01
L7N2F9 Uncharacterized protein VNVDK*VLER 9 52 1 1.04E+07 1.23E+08 11.86 8.99E-04
ADALQAGASQFETSAAK*LK 19 83 0.876 2.01E+07
ADALQAGASQFETSAAKLK* 19 85 0.539 4.61E+07
DQK*LSELDDR 10 59 1 6.10E+06 5.24E+07 8.60 2.47E-03
D9YZU5 HBB Beta-globin FFESFGDLSTPDAVMGNPK*VK* 21 60;62 0.5; 0.5 2.48E+06 2.02E+07 8.14 1.14E-03
K*VLGAFSDGLAHLDNLK 17 67 1 1.79E+06 1.02E+07 5.69 4.65E-02
P62979 RPS27A Ubiquitin-40S ribosomal protein S27a IQDK*EGIPPDQQR 13 33 1 3.34E+06 5.10E+07 15.27 1.16E-03
LIFAGK*QLEDGR 12 48 1 2.90E+09 7.33E+09 2.53 6.90E-03
F5H5D3 TUBA1C Tubulin alpha-1C chain1 VGINYQPPTVVPGGDLAK*VQR 21 370 1 2.27E+07 3.78E+07 1.67 1.38E-03
DVNAAIATIK*TK* 12 336;338 0.679;0.57 2.98E+06
Q9HCC9 ZFYVE28 Lateral signaling target protein 2 DFCVK*FPEEIR 11 87 1 7.36E+06 2.31E+07 3.14 1.50E-03
A0A024R017 HIST1H2AC Histone H2A NDEELNK*LLGR 11 96 1 3.45E+06
VTIAQGGVLPNIQAVLLPK*K* 20 119;120 0.5; 0.5 6.31E+07 1.13E+08 1.80 1.52E-03
P69905 HBA1 Hemoglobin subunit alpha AAWGK*VGAHAGEYGAEALER 20 17 1 7.09E+06
TYFPHFDLSHGSAQVK* 16 57 1 1.94E+06 2.06E+08 106.34 5.10E-03
P08670 VIM Vimentin K*LLEGEESR 9 402 1 1.70E+06 5.54E+06 3.26 5.62E-03
EK*LQEEMLQR 10 188 1 1.40E+07 2.02E+07 1.44 2.44E-01
QVDQLTNDK*AR 11 168 1 1.99E+06 2.97E+06 1.49 2.74E-01
K*LLEGEESR 9 393 1 5.54E+06
C8C504 HBB Beta-globin GTFATLSELHCDK*LHVDPENFR 22 96 1 2.04E+07 8.43E+07 4.14 5.79E-03
Q96T46 HBA2 Hemoglobin alpha 2 TYFPHFDLSHGSAQVK* 16 33 1 1.94E+06 2.03E+08 104.44 6.08E-03
Q9H582 ZNF644 Zinc finger protein 644 RSFLQQDVNK* 10 11 1 9.99E+06 4.56E+07 4.56 6.13E-03
Q9H3H9 TCEAL2 Transcription elongation factor A protein-like 2 QYK*EAIHDMNFSNEDMIR 18 154 1 3.49E+06 1.77E+07 5.07 6.49E-03
E9PL57 NEDD8-MDP1 Protein NEDD8-MDP1 TLTGK*EIEIDIEPTDKVER 19 11 1 3.50E+06 1.20E+07 3.43 6.76E-03
Q59EJ3 Heat shock 70kDa protein 1A variant MVQEAEK*YK 9 592 0.891 9.34E+05 9.36E+06 10.02 1.11E-02
D6R956 UCHL1 Ubiquitin carboxyl-terminal hydrolase CFEK*NEAIQAAHDAVAQEGQCR 22 135 1 5.86E+06
MQLK*PMEINPEMLNK 15 4 1 2.77E+06 8.80E+06 3.17 1.61E-02
P14735 IDE Insulin-degrading enzyme EVNAVDSEHEK*NVMNDAWR 19 192 1 2.05E+06 7.47E+06 3.65 1.94E-02
Q6ZUF2 cDNA FLJ43763 fis, clone TESTI2048603 FFSPPNMSVTHK*EAHERK 18 19 0.977 1.39E+08 2.50E+08 1.80 5.15E-02
P61088 UBE2N Ubiquitin-conjugating enzyme E2 N ICLDILKDK* 9 94 0.962 1.74E+07 2.27E+07 1.30 7.06E-02
D1MGQ2 HBA2 Alpha-2 globin chain AAWGK*VGAHAGEYGAEALER 20 17 1 7.09E+06
TYFPHFDLSHGSAQVK* 16 57 1 2.22E+06 1.26E+08 56.71 1.21E-01
P02042 HBD Hemoglobin subunit delta GTFSQLSELHCDK*LHVDPENFR 22 96 1 1.57E+07 2.01E+07 1.28 1.43E-01
D3DQ48 TMEM59 Transmembrane protein 59, isoform CRA_e TEDHEEAGPLPTK*VNLAHSEI 21 316 1 3.64E+06 1.31E+07 3.61 1.63E-01
K7EPI4 GFAP Glial fibrillary acidic protein GGK*STK*DGENHK* 12 10;13;19 1;1;1 2.32E+06 2.06E+07 8.87 2.34E-01
A0A024R5H9 PLEKHB1 Pleckstrin homology domain containing, family B (Evectins) member 1, isoform CRA_a LHLCAETK*DDALAWK 15 113 0.999 6.23E+06 0.00E+00 0.00 1.39E-01
B7WPA5 PLEKHB2 Pleckstrin homology domain-containing family B member 2 QNIEDK*VHMPMDCINIR 17 46 1 1.30E+07 7.08E+06 0.54 3.41E-01
Q3BBV0 NBPF1 Neuroblastoma breakpoint family member 1 VGWALDMDEIEK* 12 950 1 8.76E+06 5.34E+06 0.61 4.18E-01
A4UGR9 XIRP2 Xin actin-binding repeat-containing protein 2 WLFETQPMESLYEK* 14 934 1 1.82E+07 2.61E+07 1.44 5.39E-01
B7ZMF0 CEP192 CEP192 protein IVSPK*NSDLK 10 556 0.908 1.14E+07 1.96E+07 1.71 6.03E-01
A0N071 HBD Delta globin GTFSQLSELHCDK*LHVDPENFR 22 96 1 2.08E+07 2.20E+07 1.05 8.73E-01
A0A024QZP6 H2AFY2 Core histone macro-H2A IHPELLAK*K* 9 116;117 0.705; 0.585
A0A024R466 ITM2C Integral membrane protein 2C, isoform CRA_a ISFQPAVAGIK*GDK 14 14 0.855 1.33E+07
A0A024R6B3 TMEM63C Transmembrane protein 63C, isoform CRA_a DIEDPELIIK*HFHEAYPGSVVTR 23 239 1 3.89E+06
A0A024R7G8 RAD23A RAD23 homolog A (S. cerevisiae), isoform CRA_a LIYAGK*ILSDDVPIR 15 53 1 1.24E+07
A0A024RDB0 UBE1L2 Ubiquitin-activating enzyme E1-like 2, isoform CRA_a IDAHLNK*VCPTTETIYNDEFYTK 23 544 1 4.66E+06
A0A087WZE4 SPTA1 Spectrin alpha chain, erythrocytic 1 VNILTDK*SYEDPTNIQGK 18 79 1 6.34E+06
A0A0K0KMI2 POU5F1 POU class 5 homeobox 1 transcript variant OCT4B4 K*LGGQLGR 8 1 1 7.82E+06
A0A0U1RRH7 Histone H2A NDEELNK*LLGK 11 96 0.996
A0A140VJZ1 Testicular tissue protein Li 218 LEK*IFQNAPTDPTQDFSTQVAK 22 360 1
A0AVT1 UBA6 Ubiquitin-like modifier-activating enzyme 6 IDAHLNK*VCPTTETIYNDEFYTK 23 544 1 4.54E+06
A4D2P6 GRID2IP Delphilin GK*MGTVSK*SR 10 570;576 1; 1
A6NNT2 C16orf96 Uncharacterized protein C16orf96 SALAGK*ASR 9 819 1
A6ZKI3 FAM127A Protein FAM127A K*ESPLLNDYR 10 86 1 1.64E+07
A8K6M4 cDNA FLJ75725, highly similar to Homo sapiens vesicle transport through interaction with t-SNAREs homolog 1B (yeast) (VTI1B), mRNA ASSAASSEHFEK*LHEIFR 18 13 1 9.09E+06
B1A4G6 GH1 Growth hormone 1 isoform 1 EETQQK*SNLELLR 13 96 1 5.87E+06
B4DPP6 cDNA FLJ54371, highly similar to Serum albumin K*LVAASQAALGL 12 607 1
K*QTALVELVK 10 558 1
B4DR52 Histone H2B HAVSEGTK*AVTK 12 117 1 4.70E+07
B4E1J8 cDNA FLJ56285, highly similar to ADP-ribosylation factor-like protein 8B DLPNALDEK*QLIEK 14 193 1
B9EGU6 RC3H1 Ring finger and CCCH-type zinc finger domains 1 KEIMAQLEERK* 11 769 0.999 7.15E+06
C9JLQ8 AEBP1 Adipocyte enhancer-binding protein 1 MPPEKTK*DK* 9 7;9 0.985;1
E7EX29 YWHAZ 14-3-3 protein zeta/delta MDKNELVQKAK* 11 11 0.537 2.35E+06
K7EPF9 APOC1 Apolipoprotein C-I EFGNTLEDK*AR 11 47 1 7.55E+06
M1VKI3 SDC4-ROS1_S4;R32 Tyrosine-protein kinase receptor AGSGSQVPTEPKK* 13 105 0.554 1.53E+07
P01241 GH1 Somatotropin EETQQK*SNLELLR 13 96 1 4.23E+06
P02671 FGA Fibrinogen alpha chain EK*VTSGSTTTTR 12 448 1 4.06E+06
TVIGPDGHK*EVTK 13 476 0.86
P02768 ALB Serum albumin K*LVAASQAALGL 12 598 1
K*QTALVELVK 10 549 1
P04908 HIST1H2AB Histone H2A type 1-B/E NDEELNK*LLGR 11 96 1 3.45E+06
P09960 LTA4H Leukotriene A-4 hydrolase FSYK*SITTDDWK 12 418 1 4.10E+06
P19021 PAM Peptidyl-glycine alpha-amidating monooxygenase AFGDSEHK*LETSSGR 15 901 1
P23396 RPS3 40S ribosomal protein S3 KPLPDHVSIVEPK*DEILPTTPISEQK 26 214 1 2.84E+07
P31946 YWHAB 14-3-3 protein beta/alpha SELVQKAK* 8 13 0.672
P35212 GJA4 Gap junction alpha-4 protein ALPAK*DPQVER 11 119 1 2.26E+06
P35251 RFC1 Replication factor C KLVSETVK* 8 22 1
P45974 USP5 Ubiquitin carboxyl-terminal hydrolase 5 LEK*IFQNAPTDPTQDFSTQVAK 22 360 1 9.83E+06
P54725 RAD23A UV excision repair protein RAD23 homolog A LIYAGK*ILSDDVPIR 15 53 1 1.39E+07
LIYAGK*ILNDDTALK 15 51 0.993
P54727 RAD23B UV excision repair protein RAD23 homolog B LIYAGK*ILNDDTALK 15 51 0.993
P55072 VCP Transitional endoplasmic reticulum ATPase ASGADSK*GDDLSTAILK 17 8 1 7.91E+06
P60709 ACTB Actin, cytoplasmic 1 DSYVGDEAQSK*R 12 61 1 2.05E+06
P60866 RPS20 40S ribosomal protein S20 DTGK*TPVEPEVAIHR 15 8 1 9.48E+06
P61981 YWHAG 14-3-3 protein gamma EQLVQK*AR 8 10 1
P62979 RPS27A Ubiquitin-40S ribosomal protein S27a TITLEVEPSDTIENVK*AK 18 27 0.956 1.72E+07
MQIFVK*TLTGK 11 6 0.923
TITLEVEPSDTIENVKAK* 18 29 0.594
VDENGK*ISR 9 113 1
P83916 CBX1 Chromobox protein homolog 1 KEESEK*PR 8 109 1
Q0VDD8 DNAH14 Dynein heavy chain 14, axonemal SLLSNVSQWDTFK* 13 2919 1
Q13753 LAMC2 Laminin subunit gamma-2 ITSTFHQDVDGWK* 13 219 1 1.99E+07
Q15149 PLEC Plectin SELELTLGK*LEQVR 14 1210 1 2.72E+06
Q4LE39 ARID4B AT-rich interactive domain-containing protein 4B K*ENIK*PSLGSK* 11 462;466;472 1;1;1 2.05E+07
Q59EQ3 Nudix-type motif 6 isoform a variant LDAAAFQK*GLQGK* 13 84;89 1;1 5.22E+06
Q5I0G2 PRL Growth hormone A1 AVEIEEQTK*R 10 153 1 5.21E+06
Q5JWF2 GNAS Guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas IDVIK*QADYVPSDQDLLR 18 829 1
Q5VXU3 CHIC1 Cysteine-rich hydrophobic domain-containing protein 1 SIQK*LIEWENNR 12 176 1
Q92625 ANKS1A Ankyrin repeat and SAM domain-containing protein 1A GK*EQELLEAAR 11 3 1 8.07E+06
Q93045 STMN2 Stathmin-2 DLSLEEIQKK* 10 87 0.569 2.05E+06
DLSLEEIQK*K 10 86 0.5
Q93100 PHKB Phosphorylase b kinase regulatory subunit beta AYLQLGINEK* 10 546 1 2.91E+07
Q96AP7 ESAM Endothelial cell-selective adhesion molecule ALEEPANDIK*EDAIAPR 17 286 1
Q96EI5 TCEAL4 Transcription elongation factor A protein-like 4 EYK*EAIHDMNFSNEDMIR 18 142 1 8.59E+06
Q96N64 PWWP2A PWWP domain-containing protein 2A TGLEK*MRSGK* 10 496;501 1; 1 1.06E+07
Q9BT67 NDFIP1 NEDD4 family-interacting protein 1 TK*AEATIPLVPGR 13 83 1
Q9BUL8 PDCD10 Programmed cell death protein 10 QILSK*IPDEINDR 13 116 1 2.54E+07
Q9BWQ8 FAIM2 Protein lifeguard 2 APGTEGQQQVHGEK*K 15 25 0.822 2.50E+06
Q9H3Z4 DNAJC5 DnaJ homolog subfamily C member 5 FK*EINNAHAILTDATK 16 58 1 4.81E+06
Q9H598 SLC32A1 Vesicular inhibitory amino acid transporter DQVGGGGEFGGHDK*PK 16 113 0.832 9.03E+06
Q9NQX7 ITM2C Integral membrane protein 2C ISFQPAVAGIK*GDK 14 14 0.855 1.33E+07
Q9NV96 TMEM30A Cell cycle control protein 50A DEVDGGPPCAPGGTAK*TR 18 24 1 3.72E+06
Q9NX12 cDNA FLJ20496 fis, clone KAT08729 SGEEALIIPPDAVAVDCK*DPDDVVPVGQR 29 39 1 6.69E+06
VTFNSALAQK*EAK 13 13 0.733
Q9P0M6 H2AFY2 Core histone macro-H2A.2 IHPELLAK*K* 9 116;117 0.705; 0.585
Q9P1W3 TMEM63C Calcium permeable stress-gated cation channel 1 DIEDPELIIK*HFHEAYPGSVVTR 23 239 1 5.57E+06
Q9UBB4 ATXN10 Ataxin-10 ITSDEPLTK*DDIPVFLR 17 262 1 1.14E+07
Q9UEU0 VTI1B Vesicle transport through interaction with t-SNAREs homolog 1B ASSAASSEHFEK*LHEIFR 18 13 1 9.35E+06
Q9UF11 PLEKHB1 Pleckstrin homology domain-containing family B member 1 LHLCAETK*DDALAWK 15 113 0.999 6.23E+06
Q9UKJ5 CHIC2 Cysteine-rich hydrophobic domain-containing protein 2 SIEK*LLEWENNR 12 117 1 4.92E+06
Q9UQB3 CTNND2 Catenin delta-2 SPSIDSIQK*DPR 12 540 1 3.06E+06
Q9Y287 ITM2B Integral membrane protein 2B SGEEALIIPPDAVAVDCK*DPDDVVPVGQR 29 39 1 6.69E+06
VTFNSALAQK*EAK 13 13 0.733
S4R435 RPS10-NUDT3 Protein RPS10-NUDT3 SAVPPGADKK* 10 139 0.574 5.99E+07
SAVPPGADK*K 10 138 0.846 6.91E+07
U6FSN9 Mprip-Ntrk1 fusion gene Tyrosine-protein kinase receptor GWLTK*QYEDGQWK 13 396 1

Ratio (T/N), Ration of tumors (B) to controls(A). K*, Ubiquitinated lysine residue.