Fig. 1.

MAPK activation by GLV and wounding . Three to four week-old plants were either exposed to GLV for 1 h or wounded with a hemostat either once or 3 times across all tillers. The entire aerial-portion of the plant (one plant/tiller per time point) was collected over the course of 1 h at the times indicated. 10 W: 10 min wound sample. To determine MAPK activity, immunoblots of protein extracts were performed using anti-phospho-MAPK (Erk1/2) antibody. All experiments were repeated a minimum of three times using samples from independent experiments. Representative blots are presented