Figure 2.

Working model for ex-vivo reconstitution of autologous human ovarian tissue. Aggregation of OSCs with primitive granulosa cells, specified from iPSCs or isolated from ovarian tissue during OSC purification, enables de-novo oogenesis and folliculogenesis in the reconstituted tissue in vitro. The tissue containing new follicles is then used for orthotopic grafting to the ovaries for in-vivo growth to produce maturing follicles for oocyte aspiration or for in-vitro follicle culture to generate oocytes. Oocytes obtained from either approach are subjected to in-vitro maturation and in-vitro fertilization to generate blastocysts for embryo transfer and establishment of successful pregnancies.