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. 2019 May 28;38:226. doi: 10.1186/s13046-019-1195-y

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© The Author(s). 2019

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — miR-499a directly targets SHKBP1 expression and SHKBP1 was negatively correlated with miR-499a level in OS tissues. a Illustration of SHKBP1 3’UTR as well as the seed sequence of miR-499a showed the predicted target region on the 3’UTR of SHKBP1 mRNA. b Dual-luciferase reporter assay with co-transfection SHKBP1 3’UTR plasmids and miR-499a mimics. The relative luciferase activity was obtained by firefly luciferase activity normalized against Renilla luciferase activity. Note: Columns, mean of three individual experiments; SD,**, P < 0.01. c Down-regulation of miR-499a was observed in OS tissues compared with that in adjacent ones by qPCR (left). Up-regulation of SHKBP1 was observed in OS tissues compared with that in adjacent ones by qPCR (right). Note: Columns, mean of three individual experiments; SD,**, P < 0.01