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. 2019 May 28;7:140. doi: 10.1186/s40425-019-0622-0

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Human-derived TAM phenotype. (a) Melanoma and normal adjacent tissue were digested, stained for CX3CR1, CD163, and CD206 and analyzed by flow cytometry. Cell populations were first gated on the CD45⁺ and lineage⁻ (CD3, CD19, CD56) population. (b) SK-BR-3 in vitro generated TAMs were stained with antibodies for CX3CR1, CD163, and CD206 and analyzed by flow cytometry. Cell populations were first gated on the CD45⁺ and lineage⁻ (CD3, CD19, CD56) population