Fig. 3.

Relative abundance of 129M and 129V PrP variants in resPrP^D associated with GSS^A117V and GSS^F198S. a: GSS^A117V; b: GSS^F198S. The relative abundance of resPrP^D with M or V at residue 129 reflects the representation of the PrP mutation, which is coupled with the 129V in both GSS variants. Approximately 10–25% of resPrP^D could be identified as non-mutated (wild type) in GSS^F198S while only mutated resPrP^D could be detected in GSS^A117V. The relative populations were determined by mass spectrometry using the spectral counting method