Table 1.
Sequences of primers for reverse transcription and real-time polymerase chain reaction amplification.
| Gene | Primer sequence (5′ → 3′) | bp | |
|---|---|---|---|
| FXR | Forward primer | AAGTGACCTCCACGACCAAGC | 21 |
| (human) | Reverse primer | TCCGCTGAACGAAGGAACAT | 20 |
| FXR | Forward primer | AAGAGATGGGAATGTTGGCTG | 21 |
| (rat) | Reverse primer | CTCCCTGCATGACTTTGTTGTC | 21 |
| BSEP | Forward primer | ATGTTGACGGGATTCGCTTC | 20 |
| (human) | Reverse primer | CCACTCCAATCCCAGCAACT | 20 |
| BSEP | Forward primer | CAACTGCTGGACCGAC AACC | 20 |
| (Rat) | Reverse primer | CATCCACTGCTCCCAACAA AC | 20 |
| GAPDH | Forward primer | AGGTCCACCACTGACACGTT | 20 |
| (human) | Reverse primer | GCCTCAAGATCATCAGCAAT | 20 |
| GAPDH | Forward primer | ACAGCAACAGGGTGGTGGAC | 20 |
| (rat) | Reverse primer | TTTGAGGGTGCAGCGAACTT | 20 |