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. 2019 May 22;10:667. doi: 10.3389/fpls.2019.00667

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Copyright © 2019 Kim and Somers.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cycling pattern of PRR7-LUC protein abundance in PRR7::PRR7-LUC. (A) Image of immunoblotting of luciferase and PRR7-LUC in PRR7pro::LUC and PRR7::PRR7-LUC, respectively. 11-days old seedlings grown in 12L:12D white-light cycles were released to continuous red light (50 μmol m⁻²s⁻¹) and collected at indicated ZT time points. Protein abundances of luciferase (red arrowhead) and PRR7-LUC (blue arrowhead) were analyzed by anti-LUC immunoblotting after TCA protein precipitation. ADK protein was used as a loading control. (B) Longer exposure image of immunoblotting. (C) Relative luminescence intensity of luciferase (PRR7pro::LUC, red) and PRR7-LUC (PRR7::PRR7-LUC, blue). 7-day old seedlings grown under same conditions as used in immunoblotting were subjected to luminescence imaging analysis. PRR7-LUC is indicated by the blue arrow (A) showing a cycling pattern consistent with luminescence signals.