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. 2019 May 22;10:667. doi: 10.3389/fpls.2019.00667

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Copyright © 2019 Kim and Somers.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Interaction of elf3 mutant alleles with select known interaction partners. TAP-ELF3^WT, TAP-ELF3^A37T (elf3-14), and TAP-ELF3^P666S (elf3-13) proteins were co-expressed with either ELF3, ELF4, LUX, PIF4, phyB, GI, or TOC1, tagged with HA or GFP, in N. benthamiana. Infiltrated leaves were processed, total proteins were immunoprecipitated (human IgG) and precipitates were probed for the appropriate partner. (A) Homodimerization of ELF3 alleles. (B) Interactions with EC partners. (C) Interactions with TOC1 and GI. (D) Interactions with PIF4 and phyB. Representative immunoblots from two to three biological trials. Quantitation of these interactions are shown in Supplementary Figure S3.