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. 2019 May 22;10:566. doi: 10.3389/fphar.2019.00566

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Copyright © 2019 Shao, Gong, Qi, Wang and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PF treatment could alleviate the pathological manifestations of DN. (A) Histological observations of kidney sections stained with PAS. (B) Quantitation of CD68-positive cells, representing the recruitment of macrophages in the tissue. Macrophages were counted in randomly selected high-power (400×) fields. (C,D) Immunohistochemistry results evaluated as the average positive stained area percentage by ImagePro Plus Systems. The results are represented as the mean ± SD; n = 10 in each group. ^∗p < 0.05 and ^∗∗p < 0.01 vs. WT; ^#p < 0.05 and ^##p < 0.01 vs. WT+STZ; NS, not significant.