Figure 4.

Krox‐20 in sciatic nerves of nude mice infected by ML. Immunodetection of Krox‐20 (green: AlexaFluor 488^®) in uninfected sciatic nerve fragments (A‐C) and infected by ML (1 x 10⁶ bacilli/mL) over 6 (D‐F) and 9 mo (G‐I). Graphic of the relative frequency of Krox‐20 (J) indicates higher expression in uninfected animals compared to experimental groups. Kruskal‐Wallis test shows P < 0.01 (**) at NI x 6M and P < 0.001 (***) at NI x 9M. Images selected from five specimens/group. Bar: 30 μm. NI, uninfected; ML (6M), ML (6 mo); ML (9M), ML (9 mo) [Colour figure can be viewed at wileyonlinelibrary.com]