Figure 5.

Detection of Sox‐10 in mice Schwann cells (SC) culture. Immunodetection of Sox‐10 (red: AlexaFluor 594^®) and nuclear labelling by DAPI (blue) in SCs after 7 and 14 d. Control (A‐F) and experimental groups exposed to ML, 50 bacilli/cell (G‐L) and 100 bacilli/cell (M‐R). Graphics illustrate the relative frequency of Sox‐10 in SCs (S, T). Statistics bars identify significant differences between CTRL and ML100 after 7 d of infection. Kruskal‐Wallis test shows P < 0.05 (*) at day seven. Results are shown as medians of two independent assays, in which an average of 12 microscopic fields per group were recorded. Scale bar: 30 μm. CTRL, control; ML50, ML/SC MOI 50:1; ML100, ML/SC MOI 100:1; 7D, 7 d; 14D, 14 d [Colour figure can be viewed at wileyonlinelibrary.com]