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. 2019 May 22;10:1127. doi: 10.3389/fmicb.2019.01127

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Copyright © 2019 Zhang, Sun, Deng, Yu, Yang, Zhao, Chen and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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DENV-2 and ZIKV replicated in chicken embryos. (A,B) q-PCR standard curve for DENV-2 (A) and ZIKV (B). By using a series of diluted DENV-2 (A) or ZIKV (B) stock as standards, a formula was deduced for tissue viral load calculation. (C) The viral load of DENV-2 in various organs in chicken embryos. The chicken embryos were inoculated with 10⁴ PFU DENV-2 and samples were collected at 7 and 11 dpi, respectively. Viral RNA was extracted and quantified with qPCR. The tissue viral load was calculated with the deduced formula and expressed as PFUeq/ml. (D) The viral load of ZIKV in various organs in chicken embryos. The chicken embryos were inoculated with five PFU and 50 PFU ZIKV, and samples were collected at 7 and 11 dpi, respectively. Viral RNA was extracted and quantified with qPCR. The tissue viral loads were calculated with the deduced formula and expressed as PFUeq/ml. n = 3, ^∗P < 0.05. ^∗∗P < 0.01.