CPA induces loss of primordial follicles and primary follicles. (A) Total number of surviving primordial, primary and secondary follicles per ovary at 1, 3 and 7 days after CPA or PBS injection. Change in the number of each class of follicles over time after treatment of three different strains of PD5 mice with CPA or PBS. (B) Ratio of growing follicles to dormant follicles at 1, 3 and 7 days after treatment of three different strains of PD5 mice with CPA or PBS. (C) Level in serum AMH over time after treatment of three different strains of PD5 mice with CPA or PBS (all n = 4 each). PF, primordial follicles; PM, primary follicles; SF, secondary follicles.